Hypertension Research (2014) 37, 999–1007; doi:10.1038/hr.2014.115; published online 24 July 2014 Arterial stiffness estimation in healthy subjects: a validation of oscillometric (Arteriograph) and tonometric (SphygmoCor) techniques Open Margareta Ring1,2, Maria Jolanta Eriksson1,2, Juleen Rae Zierath3 and Kenneth Caidahl1,2 1Section of Clinical Physiology, Department of Molecular Medicine and Surgery, Karolinska Institutet, Stockholm, Sweden 2Department of Clinical Physiology, Karolinska University Hospital, Karolinska University Hospital, Stockholm, Sweden 3Section of Integrative Physiology, Department of Molecular Medicine and Surgery, Karolinska Institutet, Stockholm, Sweden Correspondence: M Ring, Department of Clinical Physiology, Karolinska Institutet, Karolinska University Hospital N2:01, Solna, 171 76 Stockholm, Sweden. E-mail: email@example.com Received 27 December 2013; Revised 29 May 2014; Accepted 6 June 2014 Advance online publication 24 July 2014 Topof page Abstract Arterial stiffness is an important cardiovascular risk marker, which can be measured noninvasively with different techniques. To validate such techniques in healthy subjects, we compared the recently introduced oscillometric Arteriograph (AG) technique with the tonometric SphygmoCor (SC) method and their associations with carotid ultrasound measures and traditional risk indicators. Sixty-three healthy subjects aged 20–69 (mean 48±15) years were included. We measured aortic pulse wave velocity (PWVao) and augmentation index (AIx) by AG and SC, and with SC also the PWVao standardized to 80% of the direct distance between carotid and femoral sites (St-PWVaoSC). The carotid strain, stiffness index and intima–media thickness (cIMTmean) were evaluated by ultrasound. PWVaoAG (8.00±2.16 m s−1) was higher (P<0.001) than PWVaoSC (6.87±1.47 m s−1), but did not differ from St-PWVaoSC (7.68±1.58 m s−1), and correlated (P<0.001) with both (r=0.54 and 0.59). St-PWVaoSC was significantly (P<0.01) higher than PWVaoAG for values below median (7.4 m s−1). PWVao by SC and AG differed significantly in females (P<0.001), but not in males (P=0.40). AIxaoAG (27.5±14.5%) was higher (P<0.001) than AIxaoSC (20.5±17.4%), but related closely (r=0.97, P<0.001). St-PWVaoSC, PWVao and AIxao by SC, and PWVao and AIxao by AG were all related to serum cholesterol and to cIMTmean (P<0.001). Arterial stiffness indices by AG and SC correlate with vascular risk markers in healthy subjects. AIxao results by AG and SC are closely interrelated, but higher values are obtained by AG. In the lower range, PWVao values by AG and SC are similar, but differ for higher values. Our results imply the necessity to apply one and the same technique for repeated studies.